Principle of SNAP/CLIP-labeling and labeling protocols. (a) SNAP-and

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Last updated 17 Jul 2024

Download scientific diagram | Principle of SNAP/CLIP-labeling and labeling protocols. (a) SNAP-and CLIP-tag fused to the protein of interest bind benzylguanine-or benzylcytosine-substrates, respectively. There are various cell-permeable fluorescent substrates and a non-fluorescent substrate available. (b) For labeling of the whole fusion protein pool cells are incubated with a fluorescent substrate, washed and fixed/imaged. (c) For age-dependent labelings cells are first incubated with the non-fluorescent blocker followed by labeling of the newly-synthesized fusion protein with a fluorescent substrate. To omit continuous labeling with the first substrate additional blocking/ labeling steps can be added. from publication: A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags | Correlative light and electron microscopy (CLEM) is a powerful approach to investigate the molecular ultrastructure of labeled cell compartments. However, quantitative CLEM studies are rare, mainly due to small sample sizes and the sensitivity of fluorescent proteins to | Protein Tagging, Electron Microscopy and Correlative Light Electron Microscopy | ResearchGate, the professional network for scientists.

Labeling mechanism of SNAPtag (a) and CLIP-tag (b) fusion proteins.

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